any charge ion or groups of ions will migrate when plate in an electric field.
Support media: could be anything......
APS: make the protein inactive
Riboflavin: active
TEMED: active the APS OR Riboflavin
Old APS == bad separation
Buffer system: dissociation system (läemmli gel)
Usually most protein bind to SDS: 1.4gr/1gr protein
Urea: Kill the interaction between proteins
Non dissociation system: No sds, native gel electrophoresis
stacking gel: make the protein concentrated
buffer considerations: velocity of migration
v: voltage gradient
m: mobility of charge species
p: portion of charged ions
Choice of pH: pH :3-10 due to hydrolytic reactions . sds complexes are not critical to pH.
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