The method is based on labeling organic compounds, including peptides and proteins, by bombarding the target (usually prepared by spray freezing the protein solution on the reactor wall chilled with liquid nitrogen) in a vacuum chamber with a beam of “hot” tritium atoms (generated through catalytic dissociation of molecular tritium at the surface of a tungsten filament heated to 2,000 K) (1). The resulting preparations are labeled to high specific activity and retain their structure and bioactivity (2、3). Labeling takes place by single collisions of tritium atoms with the target, and the intramolecular label distribution among amino acid residues is governed by their steric accessibility in the macromolecule (4). This crucial point has been verified with a quite broad range of objects (5) including complex supramolecular structures such as viruses (6、7) and ribosomes (8).
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